Analysis Note

ControlHuman thalamus tissue lysate

Application

Detect Versican using this mouse monoclonal antibody, Anti-Versican Antibody, clone 8D8.1 validated for use in IHC & western blotting.

Research CategoryCell Structure

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human cerebellum, and mouse brain and embryonic skeletal muscle.

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Versican in mouse leg muscle tissue lysate.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Versican is a large multi-domain chondroitin sulphate proteoglycan, is secreted by fibroblasts and other types of cells. It has a N-terminal hyaluronic acid (HA) binding domain. This domain has homology with the G1 and G2 domains of aggrecan, (G1 is the hyaluronic acid binding region), to cartilage link protein which stabilizes the binding of aggrecan to hyaluronic acid, and, to the hyaluronate receptor CD44. In addition, versican also has a lectin like domain, a C-terminal compliment repeat, and two EGF-like repeats, all of which can be found in aggrecan. Versican has an apparent molecular mass of ca. 1000 kDa, of which the core contributes 400 kDa from the 2409 amino acids, and the 12 -15 covalently attached chondroitin sulphate chains make up the rest.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Veriscan.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunohistochemistry in human thalamus tissue lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Versican in human thalamus tissue lysate.

Specificity

This antibody recognizes the C-terminus of Veriscan.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

372 kDa calculated. Uniprot describes three isoforms at 265 kDa, 182 kDa, and 74 kDa Versican is known to be secreted following valvular interstitual cell (VIC) injury and repair (Carthy, J., et al. (2012). Cardiovac. Pathol. 21(2):74-82.).